Microanalysis of β-cyclodextrin and glucosyl-β-cyclodextrin in human plasma by high-performance liquid chromatography with pulsed amperometric detection
1992; Elsevier BV; Volume: 201; Issue: 1 Linguagem: Inglês
10.1016/0003-2697(92)90179-b
ISSN1096-0309
AutoresYōko Kubota, Masako Fukuda, Keiko Ohtsuji, Kyoko Koizumi,
Tópico(s)Protein purification and stability
ResumoHigh-performance liquid chromatography with pulsed amperometric detection was applied to the determination of β-cyclodextrin (β-CD) and glucosyl (G)-β-CD in human plasma. They were well resolved from each other and from background components of plasma on a polymer-based reversed-phase column with 0.6% acetonitrile aqueous solution containing 1 mM sodium hydroxide as an eluent. The samples in the effluent were detected with a pulsed amperometric detector after postcolumn alkalization. The detection limits of β-CD and G-β-CD in plasma at a signal-to-noise ratio of 3 were 11 and 5 pmol, respectively.
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