Experimental Studies on Inulase
1903; University of Chicago Press; Volume: 35; Issue: 1 Linguagem: Inglês
10.1086/328314
ISSN1940-1205
Autores Tópico(s)Biofuel production and bioconversion
Resumo1. In confirmation of the results of Bourquelot, inulase was found in the fungi Aspergillus niger and Penicillium glaucum. 2. The inulase of these fungi does not pass into the culture medium; it is therefore an endo-enzyme. 3. The preparations of inulase from Aspergillus and Penicillium show similar variations in activity due to changes in the reaction of the medium. The enzyme acts most vigorously in a medium containing 0.0001 normal acid (H2SO4); greater strengths of acids are unfavorable, the enzyme being destroyed by 0.01 normal sulphuric acid. Alkalies hinder the activity of inulase, even 0.0001 normal potassium hydroxide being injurious; 0.01 normal potassium hydroxide destroys the enzyme. 4. The optimum temperature for inulase was found to be about 55⚬ C.
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