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Storage pool defect in pooled buffy coat platelet concentrates within the shelf-life period

2000; Wiley; Volume: 22; Issue: 1 Linguagem: Inglês

10.1046/j.1365-2257.2000.00267.x

1365-2257

Alfred Botchway, Nicholas A. Flores, Desmond J. Sheridan, Hannah Cohen,

Blood donation and transfusion practices

Platelet concentrate (PC) transfusions are useful for maintaining haemostasis in a variety of clinical situations. The function of transfused platelets is of critical importance, and changes on storage of buffy coat-prepared PC may influence their haemostatic potential. Total platelet adenine nucleotide content and platelet aggregation responses were studied, serially, in pooled buffy coat-derived PCs (n = 7), stored under UK recommended blood bank conditions, over the stipulated shelf-life of 5 days. Mean platelet volume (MPV), platelet counts and platelet distribution width (PDW) were also quantified. Total platelet ADP content decreased from 4.45 ± 0.78 to 3.71 ± 0.69 nmol/108 platelets (P < 0.01, day 1 versus day 5, mean ± sem) over the shelf-life period. This was associated with reduced aggregatory responses: responses (expressed as percentage of maximum height) to 5 and 10 μM ADP decreased from 10.8 ± 2.8% to 1.0 ± 1.0% (P < 0.005, 5 μM, day 1 versus day 5) and from 18.0 ± 5.4% to 4.7 ± 2.2% (P < 0.02, 10 μM, day 1 versus day 5) while the decreased responsiveness was more pronounced for 4 μg/ml of collagen: 49.0 ± 13.3% to 7.2 ± 7.1% (P < 0.01, day 1 versus day 4) and 49.9 ± 13.3% to 2.1 ± 1.9% (P < 0.001, day 1 versus day 5). These data indicate an acquired storage pool defect that is maximal by day 4 or 5 and accompanied by decreased platelet function, characterized by significant decreases in platelet aggregation responses. Addition of freeze–thawed plasma (autologous day 1) to PCs on days 2, 3, 4 and 5 did not alter the responses to ADP and collagen.