Carbohydrase activities of the rolC-gene transformed and non-transformed ginseng cultures
2002; Elsevier BV; Volume: 73; Issue: 7-8 Linguagem: Inglês
10.1016/s0367-326x(02)00231-9
ISSN1873-6971
AutoresVictor P. Bulgakov, Mikhail I. Kusaykin, G. K. Tchernoded, T. N. Zvyagintseva, Yu. N. Zhuravlev,
Tópico(s)Phytase and its Applications
ResumoThe levels of activity of β-d-glucosidase, α-d-mannosidase, α- and β-d-galactosidase, 1,3-, 1,6-, 1,4-β-d-glucanases, 1,4-α-d-glucanase, fucoidanhydrolase and agarase were measured in extracts of non-transgenic and transgenic Panax ginseng cultures transformed with the rolC gene. Significantly increased levels of activity of β- and α-d-galactosidases and 1,3-β-d-glucanase were detected in rolC-gene transformed cells, compared to the control non-transformed cells, while levels of activity of other enzymes were unchanged. These, as well as the gel-permeation experiments, revealed that transformation of ginseng cells by the rolC gene could significantly affect activity of some carbohydrases and production of their molecular forms.
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