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Effect of heat shock treatment on the production of variant testosterone-repressed prostate message-2 (TRPM-2) mRNA in culture cells

1997; Wiley; Volume: 15; Issue: 4 Linguagem: Inglês

10.1002/(sici)1099-0844(199712)15

1099-0844

Kotohiko Kimura, Kimie Asami, Mikio Yamamoto,

Nuclear Structure and Function

Cell Biochemistry and FunctionVolume 15, Issue 4 p. 251-257 Research Article Effect of heat shock treatment on the production of variant testosterone-repressed prostate message-2 (TRPM-2) mRNA in culture cells Kotohiko Kimura, Corresponding Author Kotohiko Kimura Department of Biochemistry, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, JapanDepartment of Oncology, Georgetown University Medical Center, 3970 Reservoir Rd., N.W., Washington, D.C. 20007, U.S.A.Search for more papers by this authorKimie Asami, Kimie Asami Department of Biochemistry, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, JapanSearch for more papers by this authorMikio Yamamoto, Mikio Yamamoto Department of Biochemistry, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, JapanSearch for more papers by this author Kotohiko Kimura, Corresponding Author Kotohiko Kimura Department of Biochemistry, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, JapanDepartment of Oncology, Georgetown University Medical Center, 3970 Reservoir Rd., N.W., Washington, D.C. 20007, U.S.A.Search for more papers by this authorKimie Asami, Kimie Asami Department of Biochemistry, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, JapanSearch for more papers by this authorMikio Yamamoto, Mikio Yamamoto Department of Biochemistry, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, JapanSearch for more papers by this author First published: 04 December 1998 https://doi.org/10.1002/(SICI)1099-0844(199712)15:4 3.0.CO;2-3Citations: 18AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The testosterone-repressive prostate message-2 (TRPM-2) variant mRNA lacking the exon 5 was induced in rat primary culture hepatocytes by heat shock treatment. A similar variant mRNA lacking exon 5 was also induced by heat shock treatment of the human culture cell line HepG2. On the other hand, in mouse cell line L929, heat shock treatment induced a variant TRPM-2 mRNA lacking only a small region located in exon 5. However, irrespective of the difference of mechanism of variant production, all the variant TRPM-2 mRNA species derived from each animal species encoded a putative protein constituted from the N-terminal one-third of TRPM-2 protein attached to a C-terminal TRPM-2 unrelated tail. In humans, the variant TRPM-2 species was not detected in normal tissues but was present in certain kinds of tumour cells. These results indicate that the splicing variants were induced as a direct result of heat shock treatment on cells per se and that the phenomenon of heat shock induction was observed in culture cells derived from different animal species. © 1997 John Wiley & Sons, Ltd. Citing Literature Volume15, Issue4December 1997Pages 251-257 RelatedInformation