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In Vivo Conversion of 14C-Labeled Cyclamate to Cyclohexylamine

1971; Elsevier BV; Volume: 60; Issue: 9 Linguagem: Inglês

10.1002/jps.2600600910

1520-6017

Leon Prosky, R. G. O'Dell,

Receptor Mechanisms and Signaling

To determine the effects of long-term, low-level feeding of cyclamate on cyclamate metabolism, two groups of weanling rats were fed a chow diet and a chow diet containing 0.1% calcium cyclamate, respectively, for 8 months. Twelve rats from each group were then intubated with 14C-cyclamate; urine was collected for analysis by a paper electrophoretic method which separates cyclamate, cyclohexylamine, dicyclohexylamine, and N-hydroxycyclohexylamine. Elution of the radioactive areas from the paper indicated that none of the controls converted 14C-cyclamate to additional products, but seven of 11 cyclamate-fed rats converted cyclamate to cyclohexylamine. The labeled cyclohexylamine accounted for 12–25% of the total radioactivity in urine. Two of these urine samples contained traces of radioactive dicyclohexylamine, but N-hydroxycyclohexylamine was not found. Isolated liver perfusion studies indicated that the liver is probably not the site of conversion.