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Mammalian liver contains an activity which mimics bacterial chloramphenicol acetyltransferase

1990; Elsevier BV; Volume: 1087; Issue: 3 Linguagem: Inglês

10.1016/0167-4781(90)90003-k

1879-2634

Antonio De Maio, Timothy G. Buchman,

DNA Repair Mechanisms

Rat liver extracts contain an activity which mimics Escherichia coli chloramphenicol acetyltransferase (CAT); the latter is commonly used to report transcriptional activation of chimeric genes transfected into cultured cells. Although the activities are indistinguishable by the standard thin-layer chromatography assay, alternate methods can discriminate between them. The rat CAT-like activity appears to be an integral membrane protein. It was observed in the microsomal fraction of both liver and kidney. Similarly CAT-like activities were detected in mouse, rabbit and pig liver. In addition, liver homogenates which contain the CAT-like activity also contain a heat-labile inhibitor of (authentic) bacterial CAT.