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Effect of nanotubes and apatite on growth factor release from PLLA scaffolds

2010; Wiley; Volume: 5; Issue: 6 Linguagem: Inglês

10.1002/term.339

1932-7005

Meike van der Zande, X. Frank Walboomers, Beatriz Olalde, M. J. Jurado, J.I. Álava, Otto C. Boerman, John A. Jansen,

Graphene and Nanomaterials Applications

Journal of Tissue Engineering and Regenerative MedicineVolume 5, Issue 6 p. 476-482 Research Article Effect of nanotubes and apatite on growth factor release from PLLA scaffolds Meike van der Zande, Meike van der Zande Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The NetherlandsSearch for more papers by this authorX. Frank Walboomers, X. Frank Walboomers Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The NetherlandsSearch for more papers by this authorBeatriz Olalde, Beatriz Olalde INASMET Foundation, Materials Research Centre, San Sebastian, SpainSearch for more papers by this authorMaria J. Jurado, Maria J. Jurado INASMET Foundation, Materials Research Centre, San Sebastian, SpainSearch for more papers by this authorJ. Iñaki Álava, J. Iñaki Álava INASMET Foundation, Materials Research Centre, San Sebastian, SpainSearch for more papers by this authorOtto C. Boerman, Otto C. Boerman Department of Nuclear Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The NetherlandsSearch for more papers by this authorJohn A. Jansen, Corresponding Author John A. Jansen [email protected] Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The NetherlandsRadboud University Nijmegen Medical Centre, Department of Biomaterials 309, PO Box 9101, 6500 HB Nijmegen, The Netherlands.Search for more papers by this author Meike van der Zande, Meike van der Zande Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The NetherlandsSearch for more papers by this authorX. Frank Walboomers, X. Frank Walboomers Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The NetherlandsSearch for more papers by this authorBeatriz Olalde, Beatriz Olalde INASMET Foundation, Materials Research Centre, San Sebastian, SpainSearch for more papers by this authorMaria J. Jurado, Maria J. Jurado INASMET Foundation, Materials Research Centre, San Sebastian, SpainSearch for more papers by this authorJ. Iñaki Álava, J. Iñaki Álava INASMET Foundation, Materials Research Centre, San Sebastian, SpainSearch for more papers by this authorOtto C. Boerman, Otto C. Boerman Department of Nuclear Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The NetherlandsSearch for more papers by this authorJohn A. Jansen, Corresponding Author John A. Jansen [email protected] Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The NetherlandsRadboud University Nijmegen Medical Centre, Department of Biomaterials 309, PO Box 9101, 6500 HB Nijmegen, The Netherlands.Search for more papers by this author First published: 08 September 2010 https://doi.org/10.1002/term.339Citations: 8Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract There is an evident clinical need for artificial bone restorative materials. In this respect, novel composites based on poly(L-lactic acid) (PLLA) have been described. The bone response of such polymer-based composites is usually improved by the addition of bone morphogenetic protein-2 (BMP-2). However, released BMP-2 is cleared almost immediately from the site of implantation by diffusion, whereas a prolonged retention of BMP-2 onto the scaffold has been suggested to be more favourable. Besides the ability to improve the mechanical strength and osteoconductivity of polymeric scaffolds, both carbon nanotubes (CNTs) and microhydroxyapatite (µHA) have been described to facilitate such retention of BMP-2 when incorporated into a composite scaffold. Therefore, in the current study, radiolabelled BMP-2 was loaded onto plain PLLA and composite PLLA–CNT–µHA scaffolds. Subsequently, the scaffolds were implanted subcutaneously for 5 weeks in rats and BMP-2 release was measured. Release started with an initial phase of quick release, followed by a gradual release of BMP-2. Both scaffold types comprised the same in vivo release properties for BMP-2. The bioactivity of the BMP-2 remained unaltered. It can be concluded that incorporated CNTs and µHA did not affect BMP-2 release from composite scaffold materials. Copyright © 2010 John Wiley & Sons, Ltd. References Abarrategi A, Gutierrez MC, Moreno-Vicente C, et al. 2008; Multiwall carbon nanotube scaffolds for tissue engineering purposes. Biomaterials 29(1): 94–102. Akman AC, Tigli RS, Gümüsderelioglu MS, et al. 2009; bFGF-loaded HA–Chitosan: a promising scaffold for periodontal tissue engineering. J Biomed Mater Res A 92A: 953–962. Anderson JM, Shive MS. 1997; Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev 28(1): 5–24. 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