Carboxyl‐terminal propeptide of type I collagen (c‐propeptide) modulates the action of TGF‐β on MC3T3‐E1 osteoblastic cells
2000; Wiley; Volume: 479; Issue: 3 Linguagem: Inglês
10.1016/s0014-5793(00)01900-1
ISSN1873-3468
AutoresMorimichi Mizuno, Ryuichi Fujisawa, Yoshinori Kuboki,
Tópico(s)Connective Tissue Growth Factor Research
ResumoPreviously we found that the carboxyl‐terminal propeptide of type I collagen (c‐propeptide) is a major secretory protein of MC3T3‐E1 osteoblastic cells. In this study, we found that c‐propeptide suppresses collagen synthesis and alkaline phosphatase activity of MC3T3‐E1 osteoblastic cells at the early‐differentiated stage in a dose dependent manner. Mature osteoblasts did not respond to c‐propeptide. These findings imply that c‐propeptide modulates the function of osteoblasts at an early differentiation stage. Transforming growth factor‐β (TGF‐β) is stored in bone and released from bone matrix after the resorption by osteoclasts. We investigated the effect of c‐propeptide on the action of TGF‐β, and found that it enhanced the effect of TGF‐β. We conclude that c‐propeptide is a physiological modulator of TGF‐β in bone metabolism.
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