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An HPLC method for the quantification of sterols in edible seaweeds

2004; Wiley; Volume: 18; Issue: 3 Linguagem: Inglês

10.1002/bmc.316

1099-0801

Dalia I. Sánchez‐Machado, J. López‐Hernández, P. Paseiro‐Losada, Jaime López‐Cervántes,

Marine Sponges and Natural Products

Abstract This study presents an HPLC method for the quantification of sterols in edible seaweeds. Sterols were identified by HPLC/mass spectrometry (HPLC‐MS) in positive APCI mode. The samples were saponified by refluxing with 1 m ethanolic KOH, and the non‐saponifiable fraction was extracted with hexane. Sterols were quantified by HPLC with UV detection (HPLC‐UV), on a 15 × 0.4 cm Kromasil 100 C 18 5 µm column (mobile phase 30:70 v/v methanol:acetonitrile; flow rate 1.2 mL/min; column temperature 30°C; detection wavelength 205 nm). Method repeatability for fucosterol was good (coefficient of variation 2.4%). Sterol contents were determined in canned or dried brown seaweeds ( Himanthalia elongata , Undaria pinnatifida , Laminaria ochroleuca ) and red seaweeds ( Palmaria sp., Porphyra sp.). The predominant sterol was fucosterol in brown seaweeds (83–97% of total sterol content; 662–2320 µg/g dry weight), and desmosterol in red seaweeds (87–93% of total sterol content; 187–337 µg/g dry weight). Copyright © 2004 John Wiley & Sons, Ltd.