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Biological dissolution and activity of the Allende meteorite

2013; Geological Society of America; Volume: 125; Issue: 11-12 Linguagem: Inglês

10.1130/b30791.1

1943-2674

Javiera Cervini‐Silva, Antonio Nieto‐Camacho, Hilda Cornejo-Garrido, Paz del Ángel, Nancy Patricia Rios Maya, Eduardo Palacios, J.A. Montoya, Virginia Gómez‐Vidales, María Teresa Ramírez‐Apán,

Planetary Science and Exploration

Research Article| November 01, 2013 Biological dissolution and activity of the Allende meteorite Javiera Cervini-Silva; Javiera Cervini-Silva † 1Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Artificios No. 40, sexto piso, Del. A. Obregón, México, D.F., México2Earth Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA †E-mail: jcervini@correo.cua.uam.mx. Search for other works by this author on: GSW Google Scholar Antonio Nieto-Camacho; Antonio Nieto-Camacho 3Laboratorio de Pruebas Biológicas, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México Search for other works by this author on: GSW Google Scholar Hilda Cornejo-Garrido; Hilda Cornejo-Garrido 4Posgrado en Ciencias de la Tierra Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México Search for other works by this author on: GSW Google Scholar Paz del Angel; Paz del Angel 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Search for other works by this author on: GSW Google Scholar Noel Maya; Noel Maya 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Search for other works by this author on: GSW Google Scholar Eduardo Palacios; Eduardo Palacios 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Search for other works by this author on: GSW Google Scholar José Ascención Montoya; José Ascención Montoya 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Search for other works by this author on: GSW Google Scholar Virginia Gómez-Vidales; Virginia Gómez-Vidales 6Laboratorio de Resonancia Magnética Nuclear, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México Search for other works by this author on: GSW Google Scholar María Teresa Ramirez-Apan María Teresa Ramirez-Apan 3Laboratorio de Pruebas Biológicas, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México Search for other works by this author on: GSW Google Scholar Author and Article Information Javiera Cervini-Silva † 1Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Artificios No. 40, sexto piso, Del. A. Obregón, México, D.F., México2Earth Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA Antonio Nieto-Camacho 3Laboratorio de Pruebas Biológicas, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México Hilda Cornejo-Garrido 4Posgrado en Ciencias de la Tierra Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México Paz del Angel 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Noel Maya 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Eduardo Palacios 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México José Ascención Montoya 5Dirección de Investigación y Posgrado, Instituto Mexicano del Petróleo, Eje Central Norte Lázaro Cárdenas 152, Del G.A. Madero, C.P. 07730, México D.F., México Virginia Gómez-Vidales 6Laboratorio de Resonancia Magnética Nuclear, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México María Teresa Ramirez-Apan 3Laboratorio de Pruebas Biológicas, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Del Coyoacán, C.P. 04510, México, D.F., México †E-mail: jcervini@correo.cua.uam.mx. Publisher: Geological Society of America Received: 27 Aug 2012 Revision Received: 16 Apr 2013 Accepted: 13 May 2013 First Online: 08 Mar 2017 Online ISSN: 1943-2674 Print ISSN: 0016-7606 © 2013 Geological Society of America GSA Bulletin (2013) 125 (11-12): 1865–1873. https://doi.org/10.1130/B30791.1 Article history Received: 27 Aug 2012 Revision Received: 16 Apr 2013 Accepted: 13 May 2013 First Online: 08 Mar 2017 Cite View This Citation Add to Citation Manager Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Search Site Citation Javiera Cervini-Silva, Antonio Nieto-Camacho, Hilda Cornejo-Garrido, Paz del Angel, Noel Maya, Eduardo Palacios, José Ascención Montoya, Virginia Gómez-Vidales, María Teresa Ramirez-Apan; Biological dissolution and activity of the Allende meteorite. GSA Bulletin 2013;; 125 (11-12): 1865–1873. doi: https://doi.org/10.1130/B30791.1 Download citation file: Ris (Zotero) Refmanager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentBy SocietyGSA Bulletin Search Advanced Search Abstract This paper reports on the effect of the Allende meteorite on the integrity of biological material and addresses the question whether it can induce cell damage via oxidative stress and cell mortality. The reaction mechanisms addressed herein are studied using electron-paramagnetic resonance spectroscopy (EPR), high-resolution transmission electron microscopy, scanning electron microscopy and energy dispersive spectroscopy, high-resolution X-ray diffraction, and the assays for thiobarbituric acid reactive substances (TBARS) and cell viability using 3-(4,5)-dimethylthiazol-2-yel-2,5-diphenyltetrazolium bromide (MTT bromide). As determined by the TBARS assay, Allende specimens induced cell damage via oxidative stress. The contents of TBARS in suspensions containing 1000 ppm of Allende and Fe1–xS were 6.8 ± 0.7 and 5.8 ± 0.6 nmol/mg protein, respectively. EPR experiments conducted on reaction mixtures containing Allende, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), and H2O2 showed a quartet signal, a 1:2:2:1 intensity, and hyperfine coupling constants corresponding to aN = 1.49 mT and aH = 1.49 mT, a signature of the DMPO-OH adduct. The intensity of the signal depended on the concentration of the solids in suspension, while the formation of DMPO-OH was limited by H2O2.Experiments were conducted to test for the production of the DMPO-OH adduct from ferric ions, and the plausible generation of HO•. The role of ethanol (CH3CH2OH) as scavenger of HO• in Allende-DMPO suspensions was addressed. Results showed a six-line spectra, with hyperfine coupling constants aN = 15.8 G, aH = 22.6 G, and g = 2.0059, consistent with the formation of the DMPO-CH(OH)-CH3 adduct, but not DMPO-OCH2CH3. We explain these findings as the result of formation of HO• onto (or in proximity to) the mineral surface, with CH3CH2OH competing with DMPO for HO∞, and ferric iron playing a lesser role in DMPO transformation. Our findings are congruent with reported radical-scavenging experiments for pyrite under anoxic conditions, concluding the formation of HO∞ at surface defect sites.Experiments conducted in Allende–desferrioxamine B(DFO-B) suspensions showed the inhibition of the formation of HO•, by means of decreases in the DMPO-OH adduct signal, accounted for by the reaction between Fe(II) and HO• to form Fe(III) and competing reaction mechanisms at the structural Fe centers, confirming that the production of HO∞ radicals is associated with iron centers and contributes to mineral dissolution. Small-sized magnetite domains present were recognized as catalytic sites for the production of HO∞ radicals. The γ-Fe3O4 domains present in the Allende matrix exhibited a submicron range, an elongated-hexagonal habit, and a high degree of crystallinity, supporting the presence of biogenic γ-Fe3O4. Cell viability was found to be susceptible to the distribution and atomic environment of structural Fe. You do not have access to this content, please speak to your institutional administrator if you feel you should have access.