The Comet assay for the evaluation of the genetic hazard of pollutants in cetaceans: Preliminary results on the genotoxic effects of methyl-mercury on the bottle-nosed dolphin (Tursiops truncatus) lymphocytes in vitro
1996; Elsevier BV; Volume: 32; Issue: 7 Linguagem: Inglês
10.1016/0025-326x(96)84573-5
ISSN1879-3363
Autores Tópico(s)Toxic Organic Pollutants Impact
ResumoIn this study the single cell microgel electrophoresis (Comet assay) was used to evaluate the genetic effects of methyl-mercury in dolphin (Tursiops truncatus) lymphocytes in the dose range 1–8 μg ml−1. The results of in vitro exposure showed that methyl-Hg induces DNA single-strand breaks and cytotoxicity in a dose-dependent manner. Dolphin lymphocytes have greater resistance to the genotoxic and cytotoxic effects of methyl-Hg than human and rat cells. The ecological significance of these results are discussed.
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