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Methodology of reversible proteinlabeling for ratiometric fluorescent measurement

2005; Royal Society of Chemistry; Volume: 2; Issue: 2 Linguagem: Inglês

10.1039/b515777c

1742-206X

Nobuaki Soh, Daisuke Seto, Koji Nakano, Toshihiko Imato,

Advanced Biosensing Techniques and Applications

The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.