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The quinacrine fluorescence karyotype of <i>Mus musculus</i> and demonstration of strain differences in secondary constrictions

1971; Karger Publishers; Volume: 10; Issue: 6 Linguagem: Inglês

10.1159/000130164

1424-8581

V.G. Dev, M. S. Grewal, Dorothy A. Miller, Richard E. Kouri, John J. Hutton, O. J. Miller,

Microtubule and mitosis dynamics

In the mouse, virtually every autosome pair, and the X and Y, was identified by its distinctive fluorescent banding pattern after staining with quinacrine mustard. The karyotype was characterized further by measurement of orcein-stained chromosomes and autoradiographic analysis of terminal DNA replication. The autoradiographic identification of the X and Y chromosomes was confirmed by this technique. Fluorescent karyotype analysis of inbred strains and a series of F_x hybrids involving C57BL/6J revealed a polymorphism, the presence or absence of a prominent secondary constriction on any of four different chromosomes, which was probably inherited in a simple Mendelian fashion.