Activation of a capacitative Ca 2+ entry pathway by store depletion in cultured hippocampal neurones
2000; Wiley; Volume: 470; Issue: 3 Linguagem: Inglês
10.1016/s0014-5793(00)01340-5
ISSN1873-3468
Autores Tópico(s)Nicotinic Acetylcholine Receptors Study
ResumoIntracellular Ca 2+ ([Ca 2+ ] i ) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo‐3. In the absence of external Ca 2+ , the cholinergic agonist carbachol (200 μM) and the sarco‐endoplasmic reticulum Ca 2+ pump inhibitor thapsigargin (0.4 μM) both transiently elevated [Ca 2+ ] i . A subsequent addition of Ca 2+ into the bathing medium caused a second [Ca 2+ ] i change which was blocked by lanthanum (50 μM). Taken together, these experiments indicate that stores depletion can activate a capacitative Ca 2+ entry pathway in cultured hippocampal neurones and further demonstrate the existence of such a Ca 2+ entry in excitable cells.
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