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Multiple mRNAs for human alcohol dehydrogenase (ADH): developmental and tissue specific differences

1986; Oxford University Press; Volume: 14; Issue: 9 Linguagem: Inglês

10.1093/nar/14.9.3911

1362-4962

Virginia Bilanchone, Gregg Duester, Yvonne H. Edwards, Moyro Smitha,

Eicosanoids and Hypertension Pharmacology

Human class I alcohol dehydrogenase (ADH) genes show developmental and tissue specific differences in expression at the polypeptide level.In these studies ADH expression was investigated at the RNA level. Northern blot analysis of total and poly(A)RNA from adult liver using pADHl2 probe demonstrated multiple RNA size classes of 2.6, 2.2, 1.9 and 1.6kb.In contrast, fetal liver, and fetal intestine contained only 2.6 and 1.6kb mRNA while fetal lung showed only 2.6kb mRNA.fill of these tissues showed a relative reduction in the amount of ADH mRNA present when compared to adult liver.Immunoprecipitation of in vitro translation products of adult liver RNA by polyclonal ADH antibody revealed a single polypeptide of 40,000 daltons.This result points out the homogeneity of size of class I ADH polypeptides despite mRNA size diversity.Variation in length of the 3'untranslated region probably contributes to the multiple size classes of ADH mRNA observed.