Concentrations of human cardiac phosphorus metabolites determined by SLOOP31P NMR spectroscopy
1999; Wiley; Volume: 41; Issue: 4 Linguagem: Inglês
10.1002/(sici)1522-2594(199904)41
ISSN1522-2594
AutoresMartin Meininger, Wilfried Landsch�tz, Meinrad Beer, Tobias Seyfarth, Michael Horn, Thomas Pabst, Axel Haase, Dietbert Hahn, Stefan Neubauer, Markus von Kienlin,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoHuman cardiac 31 P nuclear magnetic resonance (NMR) spectra are usually quantified in relative terms, i.e., the ratio of metabolite signals is calculated.If 31 P NMR spectroscopy of the heart is to emerge as a clinically relevant diagnostic modality, reliable quantification of absolute concentrations of 31 P metabolites is required.We applied spectral localization with optimal point spread function (SLOOP) 31 P NMR spectroscopy to measure absolute concentrations of phosphocreatine (PCr) and adenosine triphosphate (ATP) in human myocardium.The accuracy of the quantification was first validated in a phantom study.Seven healthy volunteers (aged 19-29 years) were then examined at 1.5 T using a nominal spatial resolution of 25 mL.SLOOP allowed us to obtain localized spectra from compartments anatomically matched to the left ventricular wall.The a priori knowledge of the anatomical structure was obtained from 1 H images.The spatially varying effects of saturation, off-resonance, and sensitivity were considered during the reconstruction process.Metabolites were quantified with reference to an external 31 P standard.Concentrations of 9.0 ؎ 1.2 and 5.3 ؎ 1.2 mmol/kg wet wt (mean ؎ SD, n ؍ 9) were determined for PCr and ATP in normal heart, respectively.The influence of nuclear Overhauser enhancement on metabolite quantification is dis-
Referência(s)