Metabolic roles of peptone and yeast extract for the culture of a recombinant strain ofEscherichia coli
1991; Springer Science+Business Media; Volume: 8; Issue: 3 Linguagem: Inglês
10.1007/bf01575849
ISSN0169-4146
AutoresAïcha Nancib, Christiane Branlant, Joseph Boudrant,
Tópico(s)Biofuel production and bioconversion
ResumoThe influence of complex compounds on the growth of a recombinant strain ofEscherichia coli containing the gene encoding glyceraldehyde 3-phosphate dehydrogenase, as well as the production of this enzyme have been studied. Batchwise cultures led to an accumulation of acetate, which was not utilized in a yeast extract-free medium. After glucose exhaustion, growth stopped and enzyme activity decreased. Whereas yeast extract allowed acetate assimilation and growth, peptone stabilized the enzymatic activity. The addition of both compounds resulted in optimal performances for enzyme production.
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