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Related B cell clones that populate the CSF and CNS of patients with multiple sclerosis produce CSF immunoglobulin

2011; Elsevier BV; Volume: 233; Issue: 1-2 Linguagem: Inglês

10.1016/j.jneuroim.2011.01.010

1872-8421

Birgit Obermeier, Laura Lovato, Reinhard Mentele, Wolfgang Brück, Ignasi Forné, Axel Imhof, Friedrich Lottspeich, Katherine W. Turk, Simon N. Willis, Hartmut Wekerle, Reinhard Hohlfeld, David A. Hafler, Kevin C. O’Connor, Klaus Dornmair,

Immunotherapy and Immune Responses

We investigated the overlap shared between the immunoglobulin (Ig) proteome of the cerebrospinal fluid (CSF) and the B cell Ig-transcriptome of CSF and the central nervous system (CNS) tissue of three patients with multiple sclerosis. We determined the IgG-proteomes of CSF by mass spectrometry, and compared them to the IgG-transcriptomes from CSF and brain lesions, which were analyzed by cDNA cloning. Characteristic peptides that were identified in the CSF-proteome could also be detected in the transcriptomes of both, brain lesions and CSF, providing evidence for a strong overlap of the IgG repertoires in brain lesions and in the CSF.