Preservation of Cornea by Dehydration
1955; American Medical Association; Volume: 53; Issue: 4 Linguagem: Inglês
10.1001/archopht.1955.00930010525009
ISSN2374-765X
Autores Tópico(s)Corneal surgery and disorders
ResumoThe operation of keratoplasty is frequently limited by the unavailability of donor corneal material. This report concerns preliminary experiments on the storage of cornea by dehydration and its successful use in lamellar transplantation in animals. Many attempts have been made to store corneal grafts over long periods, and various methods have been tried in animals, without success. Katzin (1947), 1 Leopold and Adler (1947), 2 and Smelser and Ozanics (1946) 3 all used rapid freezing in liquid nitrogen, with and without isopentane as an intermediate heat-transfer medium. In the method described in the first two papers, the grafts, after freezing, were dried in vacuo over P 2 O 5 at -40 C. They were reconstituted before use, in most cases, by the addition of isotonic sodium chloride solution. Smelser and Ozanics did not dry their materials but stored them at -195 C for periods of from one hour up to
Referência(s)