Portal de Periódicos da CAPES

Comparison of Healing after Cystotomy and Repair with Fibrin Glue and Sutured Closure in the Porcine Model

2008; Mary Ann Liebert, Inc.; Volume: 22; Issue: 1 Linguagem: Inglês

10.1089/end.2007.9861

1557-900X

James F. Borin, Leslie A. Deane, L Sala, Corollos S. Abdelshehid, Shannon M. White, Alfred Krebs Poulson, Farhan Khan, Robert A. Edwards, Elspeth M. McDougall, Ralph V. Clayman,

Tissue Engineering and Regenerative Medicine

Purpose: We compared healing after laparoscopic cystotomy using fibrin glue, sutures, or a combination to determine whether fibrin glue can obviate the need for sutures and whether there is any detriment when glue is used in the presence of sutures. Materials and Methods: In 24 Yorkshire pigs, a 3.5 cm vertical cystotomy was created laparoscopically and repaired as follows: Group 1—no closure; group 2—fibrin glue closure; group 3—suture repair; group 4— combined fibrin glue and suture repair. All animals had a Foley catheter for 1 week. In each group, three animals were harvested at 1 week (acute) and three animals were harvested at 6 weeks (chronic). Results: Acute: Group 1—all pigs had an unhealed defect that leaked when evaluated by cystography. Groups 2, 3, 4—mean leak pressures were 80, 97, and 60 cm H2O (P = 0.36), respectively. Mean bladder capacity was not significantly different between groups. Chronic: No leakage seen on a cystogram at 1 week; at 6 weeks, bladders were filled at ≥95 to 100 cm H2O without leakage. Histologically, there was more inflammation in the acute group v chronic group pigs. In the acute group pigs repaired with glue or suture + glue, there was more inflammation and less epithelial continuity than in the suture alone group. At 6 weeks, there was no difference between groups. Conclusion: Fibrin glue provoked an intense inflammatory response that might have delayed healing acutely, resulting in a lower burst pressure in both scenarios in which it was used (i.e., alone or in combination with sutures). However, by 6 weeks, there did not seem to be any difference between groups either clinically or histopathologically.