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Sensitive and rapid detection of Alicyclobacillus acidoterrestris using loop-mediated isothermal amplification

2011; Wiley; Volume: 91; Issue: 6 Linguagem: Inglês

10.1002/jsfa.4285

1097-0010

Jing Chen, Xiaoyan Ma, Yaowu Yuan, Wei Zhang,

Bacteriophages and microbial interactions

Journal of the Science of Food and AgricultureVolume 91, Issue 6 p. 1070-1074 Research Article Sensitive and rapid detection of Alicyclobacillus acidoterrestris using loop-mediated isothermal amplification Jing Chen, Jing Chen College of Food Science and Technology, Agricultural University of Hebei, ChinaSearch for more papers by this authorXiaoyan Ma, Xiaoyan Ma College of Food Science and Technology, Agricultural University of Hebei, ChinaSearch for more papers by this authorYaowu Yuan, Yaowu Yuan College of Food Science and Technology, Agricultural University of Hebei, ChinaSearch for more papers by this authorWei Zhang, Corresponding Author Wei Zhang [email protected] College of Food Science and Technology, Agricultural University of Hebei, ChinaCollege of Food Science and Technology, Agricultural University of Hebei, No.2596, Lekai Street (South), Baoding City, Hebei province, 071001, China.Search for more papers by this author Jing Chen, Jing Chen College of Food Science and Technology, Agricultural University of Hebei, ChinaSearch for more papers by this authorXiaoyan Ma, Xiaoyan Ma College of Food Science and Technology, Agricultural University of Hebei, ChinaSearch for more papers by this authorYaowu Yuan, Yaowu Yuan College of Food Science and Technology, Agricultural University of Hebei, ChinaSearch for more papers by this authorWei Zhang, Corresponding Author Wei Zhang [email protected] College of Food Science and Technology, Agricultural University of Hebei, ChinaCollege of Food Science and Technology, Agricultural University of Hebei, No.2596, Lekai Street (South), Baoding City, Hebei province, 071001, China.Search for more papers by this author First published: 18 February 2011 https://doi.org/10.1002/jsfa.4285Citations: 14Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract BACKGROUND: A loop-mediated isothermal amplification (LAMP) assay was developed for the rapid detection (within 2 h) of Alicyclobacillus acidoterrestris. The assay detected the species-specific DNA sequence of the 16S–23S rRNA internal transcribed spacer. RESULTS: The eight strains of A. acidoterrestris were successfully amplified, but six strains of other bacillus Acidocaldarius and 13 bacterial species other than bacillus Acidocaldarius were not. The sensitivity of the LAMP assay was at 4.50 × 10−2 cfu per tube. This sensitivity is greater than that obtained by polymerase chain reaction (PCR) assay. The LAMP assay was examined further for its ability to detect A. acidoterrestris in juice samples. The results were compared with those of conventional PCR detection. CONCLUSION: Results indicate that the proposed LAMP assay is a rapid, specific and sensitive method for detecting A. acidoterrestris. 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