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Characterization of an epithelioid cell line derived from rat small intestine: Demonstration of cytokeratin filaments

1984; Academic Press; Volume: 8; Issue: 7 Linguagem: Inglês

10.1016/0309-1651(84)90054-7

1878-240X

Jonathan Blay, Karen Brown,

Glycosylation and Glycoproteins Research

A cell line (RIE-1) of epithelioid morphology was established from rat small intestine. RIE-1 cells possess the normal rat diploid number of chromosomes (42) up to at least passage 20. Both early (less than 10) and late (greater than 20) passage cells are non-tumourigenic and do not form colonies in soft agarose. RIE-1 cells bind two epithelial-specific monoclonal antibodies. In addition, the cells exhibit a network of cytoskeletal filaments which are labelled by an antiserum to keratin. These findings provide strong evidence for an epithelial origin of the RIE-1 cell line.