Development of homospecific activity of GABA‐transaminase in the mouse cerebral cortex and cerebellum and in neurons cultured from these brain areas
1989; Wiley; Volume: 7; Issue: 1 Linguagem: Inglês
10.1016/0736-5748(89)90050-6
ISSN1873-474X
AutoresInger Schousboe, Orla M. Larsson, Arne Schousboe,
Tópico(s)Tryptophan and brain disorders
ResumoAbstract The homospecific activity of GABA‐transaminase (EC 2.6.1.19; GABA‐T) in brain or neurons was determined as a function of development in vivo or in culture by measuring the enzyme activity together with the relative amount of GABA‐T apoenzyme by the aid of a monospecific anti‐GABA‐T antibody. It was observed that both in cerebral cortex and cerebellum in vivo and in neurons cultured from these brain regions the homospecific activity of GABA‐T changed during development. By incubation of tissue extracts with similar extracts in which GABA‐T activity had been selectively and irreversibly destroyed with gamma‐vinyl GABA (Vigabatrin) it was established that this change in homospecific activity was at least partly due to the presence of an endogenous activator of GABA‐T. The results point towards a rather complex endogenous regulation of GABA‐T during development in vivo and in vitro .
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