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Characterization of Atropa belladonna L. compounds by capillary electrophoresis‐electrospray ionization‐time of flight‐mass spectrometry and capillary electrophoresis‐electrospray ionization‐ion trap‐mass spectrometry

2008; Wiley; Volume: 29; Issue: 10 Linguagem: Inglês

10.1002/elps.200700468

1522-2683

David Arraéz-Román, Gabriela Zurek, C. Bäßmann, Antonio Segura‐Carretero, Alberto Fernández‐Gutiérrez,

Insect and Pesticide Research

This short communication describes the characterization of seven tropane alkaloid compounds in Atropa belladonna L. Thus a rapid and easy CE-electrospray interface (ESI)-TOF-MS procedure is developed to analyze these compounds in a pharmaceutical preparations of A. belladonna L. leaf extract. Optimum electrophoretic separation is obtained using an alkaline solution of 60 mM ammonium acetate at pH 8.5 containing 5% isopropanol. Under the optimum CE-ESI-TOF-MS conditions several important compounds such as tropine, belladonnine, norhyoscyamine, apoatropine, hyoscyamine, 6beta-hydroxyhyoscyamine, and scopolamine have been simultaneously identified from A. belladonna L. CE-ESI-IT-MS has been used to discriminate the putative presence of littorine. The sensitivity, together with mass accuracy and true isotopic pattern of the TOF-MS, allowed the identification of a broad series of tropane alkaloid compounds present in pharmaceutical preparations of A. belladonna L. leaf extract.