Portal de Periódicos da CAPES

Consideration on equivalent chemicals in total phenolic assay of chlorogenic acid-rich plums

2004; Elsevier BV; Volume: 37; Issue: 4 Linguagem: Inglês

10.1016/j.foodres.2004.02.001

1873-7145

Ock K. Chun, Dae‐Ok Kim,

Tea Polyphenols and Effects

The spectrophotometric characteristics of chemicals used as equivalents in Folin–Ciocalteu method were evaluated. Gallic acid and (+)-catechin showed the highest response at the range of 20–100 mg/L, followed by tannic acid, and then chlorogenic acid which exhibited relatively very low absorbance. Chlorogenic acid and neochlorogenic acid showed the lower absorbance than gallic acid by 0.6 times, and it might result in underestimation of the real total phenolic content of chlorogenic acid-rich samples. Chlorogenic acid and neochlorogenic acid exhibited nearly the same spectra in a broad absorbance range, and chlorogenic acid could be appropriate for an equivalent chemical instead of neochlorogenic acid, which has been proved as a major phenolic compound in plums. The average total phenolics in 13 plum cultivars expressed by chlorogenic acid equivalent was 678.2 mg/100 g, whereas it was 368.7 mg/100 g as gallic acid equivalent. Overall, chlorogenic acid as an equivalent chemical is recommended to determine the total phenolic content of chlorogenic acid-rich plums to predict more accurate total phenolic content.