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PI4P and PI(4,5)P 2 Are Essential But Independent Lipid Determinants of Membrane Identity

2012; American Association for the Advancement of Science; Volume: 337; Issue: 6095 Linguagem: Inglês

10.1126/science.1222483

1095-9203

Gerald Hammond, Michael J. M. Fischer, Karen E. Anderson, Jon P. Holdich, Ardita Koteci, Tamás Balla, Robin F. Irvine,

Ion channel regulation and function

Phosphoinositide Contributions To study the roles of phosphoinositides in the plasma membrane of mammalian cells, Hammond et al. (p. 727 , published online 21 June; see the Perspective by Fairn and Grinstein ) engineered phosphatase molecules that could be targeted to the membrane on demand, where they would alter the concentrations of the phospholipids phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P 2 ] and phosphatidylinositol 4-phosphate (PI4P). PI4P was thought to provide a major source for the synthesis of PI(4,5)P 2 , but depletion of PI4P did not have much affect on synthesis of PI(4,5)P 2 . Instead, PI4P appears to help to establish the negative charge at the membrane and thus promote electrostatic interactions with positively charged amino acids in membrane-associated proteins and influencing function of ion channels.