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UNDERSTANDING BLADDER REGENERATION: SMOOTH MUSCLE ONTOGENY

1999; Lippincott Williams & Wilkins; Volume: 162; Issue: 3 Part 2 Linguagem: Inglês

10.1016/s0022-5347(01)68082-0

1527-3792

Hsi‐Yang Wu, Laurence S. Baskin, WENHUI LIU, YING-WU LI, Simon W. Hayward, Gerald R. Cunha,

Xenotransplantation and immune response

No AccessJournal of UrologyPapers Presented at Annual Meeting of the Section on Urology, American Academy of Pediatrics; San Francisco, California; October 17-19, 1998: Basic Science1 Sep 1999UNDERSTANDING BLADDER REGENERATION: SMOOTH MUSCLE ONTOGENY HSI-YANG WU, LAURENCE S. BASKIN, WENHUI LIU, YING-WU LI, SIMON HAYWARD, and GERALD R. CUNHA HSI-YANG WUHSI-YANG WU More articles by this author , LAURENCE S. BASKINLAURENCE S. BASKIN More articles by this author , WENHUI LIUWENHUI LIU More articles by this author , YING-WU LIYING-WU LI More articles by this author , SIMON HAYWARDSIMON HAYWARD More articles by this author , and GERALD R. CUNHAGERALD R. CUNHA More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)68082-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We determined the origin of smooth muscle cells in acellular bladder matrix grafts. Materials and Methods: A total of 15 female Sprague-Dawley rats underwent partial cystectomy and grafting with an acellular matrix derived from rat bladder. The grafts were examined 1, 2, 3 and 4 weeks after grafting by immunohistochemical studies for smooth muscle markers and by transmission electron microscopy for smooth muscle morphology. Bladder matrix and bladder epithelium recombinants were created and grafted subcutaneously and under the renal capsule in nude mice. Recombinants were examined 1, 2, 3 and 4 weeks postoperatively by immunohistochemical studies for bladder epithelium and bladder smooth muscle. Results: Smooth muscle ingrowth into acellular matrix was initially seen at 2 weeks. The immunohistochemical and electron microscopic characteristics of the cells were similar to those of fetal smooth muscle 2 weeks and newborn smooth muscle 4 weeks after grafting. Matrix epithelium recombinants displayed mature bladder epithelium with 3 to 7 layers but they did not support the ingrowth of smooth muscle cells. Conclusions: Mature bladder smooth muscle cells undergo dedifferentiation, migration and redifferentiation to repopulate an acellular matrix graft. It is unlikely that adult fibroblasts from the surrounding tissue are induced by epithelium and matrix to form smooth muscle. The contractile behavior of bladder substitute materials likely reflects the properties of the host bladder. References 1 : Metabolic complications of urinary intestinal diversion. J. Urol.1992; 147: 1199. Link, Google Scholar 2 : Regeneration of bladder urothelium, smooth muscle, blood vessels and nerves into an acellular tissue matrix. J. Urol.1996; 156: 571. Link, Google Scholar 3 : Reproduction of functional smooth muscle tissue and partial bladder replacement. Brit. J. Urol.1997; 79: 505. 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Crossref, Medline, Google Scholar From the Departments of Urology and Anatomy, University of California-San Francisco, San Francisco, CaliforniaSupported by the National Kidney Foundation of Northern California, and National Institutes of Health Grants K08 DK 02397-01, CA 59831, DK 51101 and DK 47517.© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited by Adamowicz J, Van Breda S, Tyloch D, Pokrywczynska M and Drewa T (2018) Application of amniotic membrane in reconstructive urology; the promising biomaterial worth further investigationExpert Opinion on Biological Therapy, 10.1080/14712598.2019.1556255, VOL. 19, NO. 1, (9-24), Online publication date: 2-Jan-2019. Zhang N, Qin X, Zhang J, Zhang Z, Li Y, Xie Y, Kong D, Du R, Huang X and Xu Y (2018) Bone Marrow Mesenchymal Stem Cells Accelerate the Morphological and Functional Recovery of NeovaginasArtificial Organs, 10.1111/aor.13297, VOL. 42, NO. 12, (1206-1215), Online publication date: 1-Dec-2018. 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CHUNG S, KRIVOROV N, RAUSEI V, THOMAS L, FRANTZEN M, LANDSITTEL D, KANG Y, CHON C, NG C and FUCHS G (2018) BLADDER RECONSTITUTION WITH BONE MARROW DERIVED STEM CELLS SEEDED ON SMALL INTESTINAL SUBMUCOSA IMPROVES MORPHOLOGICAL AND MOLECULAR COMPOSITIONJournal of Urology, VOL. 174, NO. 1, (353-359), Online publication date: 1-Jul-2005. Staack A, Hayward S, Baskin L and Cunha G (2005) Molecular, cellular and developmental biology of urothelium as a basis of bladder regenerationDifferentiation, 10.1111/j.1432-0436.2005.00014.x, VOL. 73, NO. 4, (121-133), Online publication date: 1-Apr-2005. Kanematsu A, Yamamoto S, Iwai-Kanai E, Kanatani I, Imamura M, Adam R, Ogawa O and Tabata Y (2005) Induction of Smooth Muscle Cell-Like Phenotype in Marrow-Derived Cells among Regenerating Urinary Bladder Smooth Muscle CellsThe American Journal of Pathology, 10.1016/S0002-9440(10)62278-X, VOL. 166, NO. 2, (565-573), Online publication date: 1-Feb-2005. 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WEFER J, SIEVERT K, SCHLOTE N, WEFER A, NUNES L, DAHIYA R, GLEASON C and TANAGHO E (2018) TIME DEPENDENT SMOOTH MUSCLE REGENERATION AND MATURATION IN A BLADDER ACELLULAR MATRIX GRAFT: HISTOLOGICAL STUDIES AND IN VIVO FUNCTIONAL EVALUATIONJournal of Urology, VOL. 165, NO. 5, (1755-1759), Online publication date: 1-May-2001. Volume 162Issue 3 Part 2September 1999Page: 1101-1105 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.Metrics Author Information HSI-YANG WU More articles by this author LAURENCE S. BASKIN More articles by this author WENHUI LIU More articles by this author YING-WU LI More articles by this author SIMON HAYWARD More articles by this author GERALD R. CUNHA More articles by this author Expand All Advertisement PDF downloadLoading ...