Artigo Revisado por pares

Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine

1999; Mary Ann Liebert, Inc.; Volume: 12; Issue: 1 Linguagem: Inglês

10.1089/vim.1999.12.1

ISSN

1557-8976

Autores

Haibin Huang, Yang Zhijun, Quanxin Xu, Zutian Sheng, Yong Xie, Weiyao Yan, Yongjin You, Sun Liyun, Zhaoxin Zheng,

Tópico(s)

T-cell and Retrovirus Studies

Resumo

In this study, we provide evidence that a recombinant fusion protein containing beta-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.

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