High-performance liquid chromatography of Dns-Amino acids and application to peptide hydrolysates
1978; Elsevier BV; Volume: 166; Issue: 2 Linguagem: Inglês
10.1016/s0021-9673(00)95640-1
ISSN1873-3778
AutoresKuo-Ting Hsu, Bruce L. Currie,
Tópico(s)Neuropeptides and Animal Physiology
ResumoThe amino acid composition of several sample peptides was analyzed by high-performance liquid chromatography on reversed-phase columns. Peptides were acid hydrolyzed and the resulting amino acids were converted to the corresponding 5-dimethylaminonaphthalenesulfunyl (Dns) derivatives and then separated on Partisil PAC. Dns-histidine was analyzed on a column of Vydac polar phase. Aqueouis acetonitrile or methanol solutions containing acetic acid were used isocratically to effect the separation of the Dns-amino acid mixtures obtained from four peptides ranging in size from three to eleven amino acids in length.
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