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Cold preserved nerve allografts: Changes in basement membrane, viability, immunogenicity, and regeneration

1998; Wiley; Volume: 21; Issue: 11 Linguagem: Inglês

10.1002/(sici)1097-4598(199811)21

1097-4598

Peter J. Evans, Susan E. Mackinnon, Allan D. Levi, Judith A. Wade, Daniel A. Hunter, Yasushi Nakao, Rajiv Midha,

Tissue Engineering and Regenerative Medicine

Muscle & NerveVolume 21, Issue 11 p. 1507-1522 Cold preserved nerve allografts: Changes in basement membrane, viability, immunogenicity, and regeneration Peter J. Evans AB, MD, PhD, FRCS(C), Peter J. Evans AB, MD, PhD, FRCS(C) Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, CanadaSearch for more papers by this authorSusan E. Mackinnon MD, FRCS(C), FACS, Corresponding Author Susan E. Mackinnon MD, FRCS(C), FACS Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri, USASuite 17424, East Pavilion, One Barnes Hospital Plaza, St. Louis, MO 63110, USASearch for more papers by this authorAllan D.O. Levi MD, PhD, FRCS(C), Allan D.O. Levi MD, PhD, FRCS(C) Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, Canada The Miami Project to Cure Paralysis, University of Miami, Miami, Florida, USASearch for more papers by this authorJudith A. Wade BSc, Judith A. Wade BSc Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, CanadaSearch for more papers by this authorDaniel A. Hunter RT, Daniel A. Hunter RT Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri, USASearch for more papers by this authorYasushi Nakao MD, PhD, Yasushi Nakao MD, PhD Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri, USASearch for more papers by this authorRajiv Midha MD, MSc, FRCS(C), Rajiv Midha MD, MSc, FRCS(C) Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, CanadaSearch for more papers by this author Peter J. Evans AB, MD, PhD, FRCS(C), Peter J. Evans AB, MD, PhD, FRCS(C) Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, CanadaSearch for more papers by this authorSusan E. Mackinnon MD, FRCS(C), FACS, Corresponding Author Susan E. Mackinnon MD, FRCS(C), FACS Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri, USASuite 17424, East Pavilion, One Barnes Hospital Plaza, St. Louis, MO 63110, USASearch for more papers by this authorAllan D.O. Levi MD, PhD, FRCS(C), Allan D.O. Levi MD, PhD, FRCS(C) Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, Canada The Miami Project to Cure Paralysis, University of Miami, Miami, Florida, USASearch for more papers by this authorJudith A. Wade BSc, Judith A. Wade BSc Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, CanadaSearch for more papers by this authorDaniel A. Hunter RT, Daniel A. Hunter RT Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri, USASearch for more papers by this authorYasushi Nakao MD, PhD, Yasushi Nakao MD, PhD Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri, USASearch for more papers by this authorRajiv Midha MD, MSc, FRCS(C), Rajiv Midha MD, MSc, FRCS(C) Division of Orthopaedic Surgery, University of Toronto, Toronto, Ontario, CanadaSearch for more papers by this author First published: 07 December 1998 https://doi.org/10.1002/(SICI)1097-4598(199811)21:11 3.0.CO;2-WCitations: 92AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Rat sciatic nerve graft segments were harvested and pretreated by either placement in the University of Wisconsin Cold Storage Solution at 5°C and storage from 1 to 26 weeks, or repeatedly freezing (−40°C) and thawing (20°C). Following pretreatment, grafts were transplanted as either syngeneic or allogeneic nerve grafts. Storage and freeze–thawing did not affect the Schwann cell basal lamina or laminin distribution of the peripheral nerve. Graft cell viability decreased with increasing time of storage, with some viable cells detectable even after 3 weeks of storage. Freeze–thawed grafts were not viable. Increasing time of storage led to decreasing immune response and graft rejection, but improved regeneration. Freeze–thawed and 26-week stored allografts were nonimmunogenic and rejection was not seen, but regeneration was delayed compared to autografts. 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