Kinetic expression for human cell growth in a suspension culture system
1986; Elsevier BV; Volume: 64; Issue: 4 Linguagem: Inglês
10.1016/0385-6380(86)90130-5
ISSN1878-2108
AutoresMasahito Taya, Takashi Mano, Takeshi Kobayashi,
Tópico(s)Biopolymer Synthesis and Applications
ResumoThe effects of lactate and ammonium concentrations on the growth of HL-60 and RPMI 8226 human cells were kinetically evaluated in a suspension culture system. The specific growth rates of the cells were expressed as the following equation with the terms of the inhibitory effects owing to lactate, ammonium, and glucose. mu;=mu;mS{(Ks+S)(1+P1k1+P2k2)+S 2k3} On the basis of this equation, it was possible to stimulate the culture processes of the human cells.
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