A protein chemical analysis of the heterogeneity of the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Zea mays
1996; Elsevier BV; Volume: 113; Issue: 1 Linguagem: Inglês
10.1016/0168-9452(95)04265-2
ISSN1873-2259
AutoresDjamileh Wagner, Johann Salnikow, Albrecht Otto, Bernd Thiede, Joachim Vater,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoThe small subunit (S) of ribulose-1,5-bisphosphate carboxylase/oxygenase from Zea mays is coded by a multi-gene family and expressed as a mixture of polypeptides. Three isoforms of this mixture can be separated by high performance liquid chromatography on reversed phase. The two major components—isoform 1 and isoform 2—have been analyzed by tryptic digestion and selected peptides characterized by mass spectrometry and chemical sequencing. Evidence is obtained that the polypeptides differ in their primary structure.
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