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CCD camera imaging for the chemiluminescent detection of enzymes using new ultrasensitive reagents

1994; Wiley; Volume: 9; Issue: 3 Linguagem: Inglês

10.1002/bio.1170090309

1099-1271

Hashem Akhavan‐Tafti, A. Paul Schaap, Zahara Arghavani, Renuka DeSilva, Robert A. Eickholt, R S Handley, Barry A. Schoenfelner, Katsuaki Sugioka, Yumiko Sugioka,

Analytical Chemistry and Sensors

Abstract We have designed and constructed an inexpensive imaging system based on charge coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen® chemiluminescent reagents. We also report the development of two new chemiluminescent reagents, Lumi‐Phos® Plus and Lumigen PS. Lumi‐Phos Plus is an ehanced formulation for the rapid detection of alkaline phosphatase on membranes and in solution. It provides excellent images in blotting applications with exposures of under a minute. Lumigen PS represents a new generation of peroxidase detection reagents. The wide dynamic range with excellent linearity, higher signal and lower background than other chemiluminescent reagents make Lumigen PS of unsurpassed value in enzyme‐linked immunoassays and nuclic acid probe assays using HRP conjugates.