Construction and characterization of a fusion protein with epidermal growth factor and the cell‐binding domain of fibronectin
1992; Wiley; Volume: 298; Issue: 2-3 Linguagem: Inglês
10.1016/0014-5793(92)80037-h
ISSN1873-3468
AutoresYasutoshi Kawase, Yoichi Ohdate, Tomoko Shimojo, Yuki Taguchi, Fusao Kimizuka, Ikunoshin Kato,
Tópico(s)Peptidase Inhibition and Analysis
ResumoAn efficient expression system was constructed for C‐EGF, a fusion protein made of a fragment of the cell‐binding domain of human fibronectin (FN) bound with epidermal growth factor (EGF), C‐EGF was produced in Escherichia coli HB101 cells carrying the recombinant plasmid pCE102 as inclusion bodies, which were solubilized and refolded after purification. C‐EGF had both cell‐adhesive and EGF activities, so it might be more effective than EGF in therapeutic applications. This fusion system would be useful for the construction of a recombinant drug delivery system for cells that have fibronectin receptors (integrins).
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