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The selective degradation of pyrimidines in nucleic acids by permanganate oxidation

1967; Elsevier BV; Volume: 29; Issue: 4 Linguagem: Inglês

10.1016/0006-291x(67)90521-9

1090-2104

Hikoya Hayatsu, T. UKITA,

Synthesis and Characterization of Heterocyclic Compounds

In our current studies on the chemical modifications of nucleic acids, we have reported on the selective reaction of semicarbazide derivatives with cytidine residues (Hayatsu and Ukita, 1964, Hayatsu and Ukita, 1966; Hayatsu et al., 1966; Kikugawa et al., 1967a, Kikugawa et al., 1967b). In this paper we describe a procedure by which pyrimidines in the single stranded region of nucleic acids are degraded selectively. Furthermore, reaction conditions are presented whereby thymidylic acid is exclusively attacked while other deoxyribonucleotides are virtually intact. This procedure consists of the permanganate oxidation of the nucleic acid under relatively mild reaction conditions of pH 6.7 and 0°C.