Cytokine gene polymorphisms in Colombian patients with different clinical presentations of tuberculosis
2005; Elsevier BV; Volume: 86; Issue: 1 Linguagem: Inglês
10.1016/j.tube.2005.03.001
ISSN1873-281X
AutoresMarcela I. Henao, Carlos Montes, Sara París, Luis F. García,
Tópico(s)Immunodeficiency and Autoimmune Disorders
ResumoTuberculosis (TB) has different clinical presentations. Pulmonary TB affects only the lungs and exhibits variable anti-mycobacterial immune responses. Pleural TB is a localized disease with a strong immune response. Miliary TB is a disseminated form with poor immune response. Cytokines play a pivotal role in anti-mycobacterial response and may determine the type of TB. Thus, gene polymorphisms associated with cytokine production may be associated with clinical presentations of TB. In this study, 54 tuberculin-negative healthy controls, 81 tuberculin-positive healthy controls, 140 patients with pulmonary TB, 30 with pleural TB and 20 with miliary TB were studied. Single nucleotide polymorphisms were typed for tumour necrosis factor-α, interferon-γ (IFN-γ), transforming growth factor-β1, interleukin-10 (IL-10) and interleukin-6 by sequence-specific primer polymerase chain reaction (SSP-PCR). Allelic, genotypic and haplotypic associations with clinical forms of TB were evaluated. IL-10 −1082 A/A genotype and IFNγ+874 T allele were associated with pleural TB. Seventy-five extended genotypes were found; two differed between patients and controls, and two between groups of patients. Results suggest that IL-10 low-producer polymorphism and IFN-γ high-producer polymorphism are associated with pleural TB.
Referência(s)