Quantitation of six androgens by combined high performance liquid chromatography and radioimmunoassay
1982; Elsevier BV; Volume: 40; Issue: 4 Linguagem: Inglês
10.1016/0039-128x(82)90014-9
ISSN1878-5867
AutoresSusan Cook, N.C. Rawlings, RICHARD I. KENNEDY,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoA technique was developed to separate six androgens (testosterone, dihydrotestosterone, androstenedione, 5α-androstane-3 α, 17β-diol, 5α-androstane-3β, 17β-diol, and androsterone) by high performance liquid chromatography prior to quantitation by specific radioimmunoassay systems. Methanol:water (60:40 v:v) was used as the solvent system with a C18 reversed-phase column. The method was verified and used to quantitate the androgens in serum from adult rams bled every 20 minutes for 6 hours and yearling bulls bled every 30 minutes for 8 hours. Concentrations of all 6 androgens varied in an episodic manner with testosterone being the dominant androgen.
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