Temperature-sensitive mutants of human adenovirus type 12
1971; Elsevier BV; Volume: 45; Issue: 3 Linguagem: Inglês
10.1016/0042-6822(71)90206-6
ISSN1096-0341
AutoresUlla Lundholm, Walter Doerfler,
Tópico(s)Animal Disease Management and Epidemiology
ResumoThe Indian deer Muntiacus muntjak has the lowest chromosome number reported for mammals, six for the female and seven for the male animal. Adenovirus type 2 (Ad2) can replicate efficiently in these cells. When inoculated at low multiplicity, however, Ad2 establishes a persistent infection. The viral DNA persists in these cells apparently in a free form and replicates continuously. Tests for viral antigens and infectious center assays have revealed that about 1 to 2% of the cells produce Ad2. The persistent infection can be abolished by treating the infected cells with rabbit anti-Ad2 serum. As shown by the Southern blotting technique, the viral DNA is lost from the cells which were cured by antiserum treatment. Judging from the unabated replication of Sindbis virus or vesicular stomatitis virus in persistently Ad2-infected Muntiacus muntjak (M.m.) cells, the persistence of Ad2 is not accompanied by the production of interferon. Nor is there evidence for an Ad2-specific inhibitory factor, since persistently infected cells can be superinfected with high multiplicities of Ad2. Adenovirus type 12 (Ad 12) cannot multiply in M.m. cells. A very low level of replication of unit length Ad12 DNA can be detected. M.m. cells abortively infected with Ad12 continue to grow, and the viral DNA is eventually lost from the bulk of the cells. Ad12 infection leads to chromosomal abnormalities in M.m. cells. At 24 hr postinfection, 30–40% of the cells could be shown to be T antigen positive by immune fluorescence.
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