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Distribution of N-methyl-D-aspartate and non-N-methyl-D-aspartate glutamate receptor subunits on respiratory motor and premotor neurons in the rat

1997; Wiley; Volume: 389; Issue: 1 Linguagem: Inglês

10.1002/(sici)1096-9861(19971208)389

1096-9861

Deborah D. Robinson, Howard H. Ellenberger,

Neuroendocrine regulation and behavior

Journal of Comparative NeurologyVolume 389, Issue 1 p. 94-116 Distribution of N-methyl-D-aspartate and non-N-methyl-D-aspartate glutamate receptor subunits on respiratory motor and premotor neurons in the rat Deborah Robinson, Deborah Robinson Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, CanadaSearch for more papers by this authorHoward Ellenberger, Corresponding Author Howard Ellenberger [email protected] Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, CanadaDepartment of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, CanadaSearch for more papers by this author Deborah Robinson, Deborah Robinson Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, CanadaSearch for more papers by this authorHoward Ellenberger, Corresponding Author Howard Ellenberger [email protected] Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, CanadaDepartment of Anatomy and Neurobiology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, CanadaSearch for more papers by this author First published: 06 December 1998 https://doi.org/10.1002/(SICI)1096-9861(19971208)389:1 3.0.CO;2-9Citations: 37AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Glutamate is required for the transmission of inspiratory drive in respiratory premotor and motor neurons. The glutamate receptors (GluRs) responsible for this essential function have yet to be anatomically characterized. We mapped the GluR subtypes expressed by respiratory premotor and motor neurons by using combined immunohistochemistry and retrograde labeling in adult rats. Phrenic motoneurons and bulbospinal ventral respiratory group (VRG) neurons were retrogradely labeled and immunolabeled with subunit-specific antibodies against the N-methyl-D-aspartate (NMDA) receptor subtype (NMDAR1) and the non-NMDA receptor subtypes, α-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA; GluR1, GluR2/3, GluR4) and kainate (GluR5–7). Phrenic motoneurons and bulbospinal VRG neurons showed positive immunolabeling for all five GluR subunits. These results support the hypothesis that NMDA and non-NMDA receptor subtypes underlie the excitation of bulbospinal VRG neurons and phrenic motoneurons. Furthermore, immunolabeling for each receptor subtype demonstrated a unique distribution along the neuronal membrane. Immunoreactivity for AMPA receptor subunits was distributed throughout somata and proximal dendrites, NMDAR1 subunit immunolabeling was localized to somata, and GluR5–7 subunit immunolabeling was confined largely to dendrites. The differential distribution of AMPA, kainate, and NMDA receptors on the somal and dendritic surface of respiratory neurons suggests that the location of glutamatergic synapses along the neuronal surface is an important determinant of glutamate-mediated postsynaptic currents. Consequently, different patterns of glutamatergic excitation of respiratory neurons could be achieved by selective activation of different profiles of GluR subtypes on different portions of the neuronal membrane. J. Comp. 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Citing Literature Volume389, Issue18 December 1997Pages 94-116 ReferencesRelatedInformation