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A platelet aggregation inhibitor phospholipase a2 from russell's viper (vipera russelli) venom: Isolation and characterization

1996; Elsevier BV; Volume: 34; Issue: 10 Linguagem: Inglês

10.1016/0041-0101(96)00033-5

1879-3150

Bhaskarjyoti Prasad, Kempaiah Kemparaju, Kandarp Bhatt, T. Veerabasappa Gowda,

Ion channel regulation and function

Vipera russelli venom is reported to contain multiple forms of phospholipase A2 (PLA2). Three PLA2s (VRV-PL-V, VRV-PL-VI and VRV-PL-VIIIa) have been purified and characterized in this laboratory. A PLA2(VRV-PL-IIIb) inhibiting platelet aggregation was purified from the same venom in two steps involving CM-Sephadex C-25 column chromatography followed by gel filtration on Sephadex G-50. VRV-PL-IIIb is a basic (pI 7.3-7.7) isoenzyme, with a mol. wt between 14,000 and 15,000. It induced neurotoxic symptoms in experimental mice with an i.p. LD50 of 5.2 mg/kg body weight. VRV-PL-IIIb inhibits ADP-induced platelet aggregation in a dose-dependent manner. It induced oedema in the foot pads of mice and is devoid of anticoagulant, myotoxic and direct haemolytic activities. Antibodies to VRV-PL-IIIb showed a single precipitin line against the antigen as well as whole venom.