Portal de Periódicos da CAPES

Multilocus sequence analysis reveals the genetic diversity of European fruit tree phytoplasmas and supports the existence of inter-species recombination

2010; Microbiology Society; Volume: 157; Issue: 2 Linguagem: Inglês

10.1099/mic.0.043547-0

1465-2080

Jean Luc Danet, Gulnara Balakishiyeva, Agnès Cimerman, Nicolas Sauvion, Véronique Marie-Jeanne, Gérard Labonne, A. Laviña, A. Batlle, Ivana Križanac, Dijana Škorić, P. Ermacora, Çiğdem Ulubaş Serçe, Kadriye Çağlayan, W. Jarausch, Xavier Foissac,

Insect symbiosis and bacterial influences

The genetic diversity of three temperate fruit tree phytoplasmas 'Candidatus Phytoplasma prunorum', 'Ca. P. mali' and 'Ca. P. pyri' has been established by multilocus sequence analysis. Among the four genetic loci used, the genes imp and aceF distinguished 30 and 24 genotypes, respectively, and showed the highest variability. Percentage of substitution for imp ranged from 50 to 68 % according to species. Percentage of substitution varied between 9 and 12 % for aceF, whereas it was between 5 and 6 % for pnp and secY. In the case of 'Ca P. prunorum' the three most prevalent aceF genotypes were detected in both plants and insect vectors, confirming that the prevalent isolates are propagated by insects. The four isolates known to be hypo-virulent had the same aceF sequence, indicating a possible monophyletic origin. Haplotype network reconstructed by eBURST revealed that among the 34 haplotypes of 'Ca. P. prunorum', the four hypo-virulent isolates also grouped together in the same clade. Genotyping of some Spanish and Azerbaijanese 'Ca. P. pyri' isolates showed that they shared some alleles with 'Ca. P. prunorum', supporting for the first time to our knowledge, the existence of inter-species recombination between these two species.