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Bovine serum albumin adsorption on nano-rough platinum surfaces studied by QCM-D

2008; Elsevier BV; Volume: 66; Issue: 1 Linguagem: Inglês

10.1016/j.colsurfb.2008.05.010

1873-4367

Alireza Dolatshahi‐Pirouz, Kristian Rechendorff, Mads Bruun Hovgaard, Morten Foss, J. Chevallier, Flemming Besenbacher,

Adhesion, Friction, and Surface Interactions

The adsorption of bovine serum albumin (BSA) on platinum surfaces with a root-mean-square roughness ranging from 1.49 nm to 4.62 nm was investigated using quartz crystal microbalance with dissipation (QCM-D). Two different BSA concentrations, 50 μg/ml and 1 mg/ml, were used, and the adsorption studies were complemented by monitoring the antibody interaction with the adsorbed BSA layer. The adsorption process was significantly influenced by the surface nano-roughness, and it was observed that the surface mass density of the adsorbed BSA layer is enhanced in a non-trivial way with the surface roughness. From a close examination of the energy dissipation vs. frequency shift plot obtained by the QCM-D technique, it was additionally observed that the BSA adsorption on the roughest surface is subject to several distinct adsorption phases revealing the presence of structural changes facilitated by the nano-rough surface morphology during the adsorption process. These changes were in particular noticeable for the adsorption at the low (50 μg/ml) BSA concentration. The results confirm that the nano-rough surface morphology has a significant influence on both the BSA mass uptake and the functionality of the resulting protein layer.