A new product of linoleic acid oxidation by a flaxseed enzyme
1966; Elsevier BV; Volume: 23; Issue: 4 Linguagem: Inglês
10.1016/0006-291x(66)90740-6
ISSN1090-2104
Autores Tópico(s)Nitrogen and Sulfur Effects on Brassica
ResumoAlthough the products of lipoxidase activity have been known for some time, the function of this enzyme in plant metabolism is not known (Tappel, 1963). This paper reports the isolation of an enzyme from flaxseed which utilizes the product of lipoxidase as its substrate, and the tentative identification of the resultant product. The reaction catalyzed by the flaxseed enzyme is shown below. The reaction catalyzed by the flaxseed enzyme is essentially an isomerization of an unsaturated hydroperoxide to a ketohydroxy compound. For this reason the trivial name, hydroperoxide isomerase, is suggested. Linoleic acid was first incubated with soybean lipoxidase and then with the flaxseed preparation. After acidification and extraction, the product was identified by thin-layer chromatography (TLC), infra-red (IR) and ultraviolet (UV) spectroscopy, nuclear magnetic resonance (NMR) analysis and periodate oxidation.
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