Portal de Periódicos da CAPES

Enhanced neuronal expression in reaggregating cells of mouse cerebellum cultured in the presence of poly-l-lysine

1978; Elsevier BV; Volume: 64; Issue: 2 Linguagem: Inglês

10.1016/0012-1606(78)90076-3

1095-564X

Leslie P. Kozak, John J. Eppig, Doris Dahl, A. Bignami,

Supramolecular Self-Assembly in Materials

Both neuronal and glial cell differentiation occur in reaggregating cell cultures of mouse cerebellums, as evidenced by electron microscopy and immunofluorescence to the glial fibrillary acidic protein (GFA). However, after the initial 10 days in culture a process occurs in which the neuronal cells degenerate while glial cells predominate. We have found that when poly-l-lysine is added to the culture medium either for the entire culture period or during the latter days of culture, i.e., Days 4 through 10, the neuronal character is stabilized, as evidenced by acetylcholin-esterase levels and electron microscopy, while the gliosis is inhibited. Culturing reaggregating cells in poly-l-lysine containing medium from Days 0 through 4 has no inhibitory influence on the gliosis observed on Day 10. Cerebellar cells cultured as monolayers on plastic surfaces coated with poly-l-lysine express an intense immunofluoresence with antisera to GFA as do cells grown on uncoated flasks. The data suggest that poly-l-lysine in reaggregating cell cultures stabilizes the neuronal cells by some unknown mechanism. It is postulated that a stable neuronal population reduces the trend toward gliosis in cerebellar aggregates.