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Altered surface marker expression and function of G‐CSF‐induced neutrophils from test subjects and patients under chemotherapy

1994; Wiley; Volume: 87; Issue: 1 Linguagem: Inglês

10.1111/j.1365-2141.1994.tb04866.x

1365-2141

Karsten Spiekermann, Andreas Emmendoerffer, J. Elsner, Evelin Raeder, Marie‐Luise Lohmann‐Matthes, Arndt Prahst, Hartmut Link, Mathias Freund, Karl Welte, Joachim Roesler,

Erythrocyte Function and Pathophysiology

Summary We have previously reported an altered surface marker expression and chemotaxis of G‐CSF‐induced neutrophils from patients with severe congenital neutropenia. However, effects of G‐CSF and influence of the underlying disease on neutrophils could not be discerned. In this study we have evaluated the effects of G‐CSF on neutrophil phenotype and function in patients under chemotherapy and in healthy test subjects. We found a significantly enhanced expression of FcγRI, CD14 and CD54 and a decrease in the level of FcγRIII during G‐CSF treatment. In addition, motility of G‐CSF‐induced neutrophils was significantly decreased. The effects were seen in patients under cytotoxic chemotherapy and in healthy test subjects. Surface marker alterations and neutrophil motility were affected by G‐CSF administration in a dose‐dependent manner. Kinetic studies on neutrophils from healthy test subjects demonstrated that all effects could be seen after a single administration of 300 μg G‐CSF and began to appear within 4 h. Release of partially immature neutrophils from the bone marrow and indirect activation of these cells by G‐CSF are discussed as possible reasons for the findings presented. They demonstrate that G‐CSF has profound effects on neutrophil phenotype and function in vivo which might have clinical implications.