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Semiautomated measurement of rapidly hydrolyzed DNA in the diagnosis of mammary carcinoma

1986; Wiley; Volume: 57; Issue: 1 Linguagem: Inglês

10.1002/1097-0142(19860101)57

1097-0142

A. M. Sincock,

RNA and protein synthesis mechanisms

CancerVolume 57, Issue 1 p. 1-5 ArticleFree Access Semiautomated measurement of rapidly hydrolyzed DNA in the diagnosis of mammary carcinoma Andrew M. Sincock BSc, PhD, Corresponding Author Andrew M. Sincock BSc, PhD Department of Biology, University of Southampton, Southampton, United KingdomDepartment of Biology, Medical and Biological Sciences Building, Bassett Crescent East, Southampton S093TU, United Kingdom===Search for more papers by this author Andrew M. Sincock BSc, PhD, Corresponding Author Andrew M. Sincock BSc, PhD Department of Biology, University of Southampton, Southampton, United KingdomDepartment of Biology, Medical and Biological Sciences Building, Bassett Crescent East, Southampton S093TU, United Kingdom===Search for more papers by this author First published: 1 January 1986 https://doi.org/10.1002/1097-0142(19860101)57:1 3.0.CO;2-TCitations: 4AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract A modified Feulgen procedure combined with integrating microdensitometry was used to quantify the rapidly hydrolyzed DNA fractions in nuclei obtained from primary carcinomas of the human breast. The method, which was semiautomated by interfacing the microdensitometer with a Research machines 380Z microcomputer (Research machines, Ltd., Oxford, UK), could distinguish malignant carcinomas from benign breast lesions. It is proposed that the semiautomated assessment of rapidly hydrolyzed DNA fractions could serve as a reliable additional parameter on which to diagnose breast malignancies. References 1 Stenkvist B, Bengtsson E, Eriksson O, Jarkrans T, Nordin B, Westman-Naeser S. Histopathological systems of breast cancer classification: Reproductibility and clinical significance. J Clin Pathol 1983; 36: 392–398. 2 Schiodt T. Breast carcinoma: A histologic and prognostic study of 650 followed-up cases. 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