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Therapeutic drug monitoring for sirolimus in whole blood of organ transplants by high-performance liquid chromatography with ultraviolet detection

2003; Elsevier BV; Volume: 1031; Issue: 1-2 Linguagem: Inglês

10.1016/j.chroma.2003.10.121

1873-3778

Miguel Ángel Campanero, Ernesto Cardenas, Belén Sádaba, E. García-Quetglás, Maria José Muñoz-Juarez, Isabel Gil-Aldea, David Pazo, José Ramón Azanza Perea, Jesús Honorato,

Organ Transplantation Techniques and Outcomes

We developed and validated an accurate, sensitive, precise and rapid HPLC method with UV detection for the determination of sirolimus in blood samples from renal, cardiac and hepatic transplants. This method overcomes most of the problems related to previously published assays using a narrow-bore column with base deactivated C18 reversed phase. Whole blood samples were purified by a combination of a precipitating blood matrix with zinc sulphate and a single step liquid–liquid extraction with acetone and 1-chlorobutane. Calibration curves (range 2.5–150 ng/ml), were linear with coefficients of correlation better than 0.996. The relative standard deviation was determined to be less than 8%. The present method has also been validated by a reference laboratory (St. George’s Hospital Medical School, London, UK). More of 300 clinical samples have been analysed with this method.