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BIBTEX RIS

Validação de método analítico livre de acetonitrila para análise de microcistinas por cromatografia líquida de alta eficiência

2013; Brazilian Chemical Society; Volume: 36; Issue: 8 Linguagem: Inglês

10.1590/s0100-40422013000800019

ISSN

1678-7064

Autores

Sérvio Túlio Alves Cassini, Paulo Wagner Pereira Antunes, Regina Keller,

Tópico(s)

Amino Acid Enzymes and Metabolism

Resumo

LIQUID CHROMATOGRAPHY.Blooms of cyanobacteria represent a public health risk due to their cyanotoxins such as microcystins.Liquid chromatography techniques to separate and quantify microcystins invariably use acetonitrile as the organic component of the mobile phase.The price and availability of acetonitrile together with its elevated toxicity encourage the validation of acetonitrile-free methods of microcystin analysis.In this work, methanol was employed as the organic solvent of the mobile phase and the validation method was performed with different environmental water samples.The method showed limits of detection between 0.17 and 0.25 μg/L and of quantification between 0.55 and 0.82 μg/L for the microcystin variants: -RR, -YR, -LR, -LA.

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