Portal de Periódicos da CAPES

Dynamin 2 regulates T cell activation by controlling actin polymerization at the immunological synapse

2005; Nature Portfolio; Volume: 6; Issue: 3 Linguagem: Inglês

10.1038/ni1168

1529-2916

Timothy S. Gomez, Michael J. Hamann, Sean McCarney, Doris N. Savoy, Casey M. Lubking, Michael P. Heldebrant, Christine M. Labno, D J McKean, Mark A. McNiven, Janis K. Burkhardt, Daniel D. Billadeau,

Immune Cell Function and Interaction

Actin reorganization at the immunological synapse is required for the amplification and generation of a functional immune response. Using small interfering RNA, we show here that dynamin 2 (Dyn2), a large GTPase involved in receptor-mediated internalization, did not alter antibody-mediated T cell receptor internalization but considerably affected T cell receptor–stimulated T cell activation by regulating multiple biochemical signaling pathways and the accumulation of F-actin at the immunological synapse. Moreover, Dyn2 interacted directly with the Rho family guanine nucleotide exchange factor Vav1, and this interaction was required for T cell activation. These data identify a functionally important interaction between Dyn2 and Vav1 that regulates actin reorganization and multiple signaling pathways in T lymphocytes.